EFFECTS OF IONIZING RADIATION ON THE FINE STRUCTURE AND FUNCTION OF THE INTESTINAL EPITHELIUM OF THE MOUSE. I. VILLUS EPITHELIUM
The effects of ionizing radiation on villus epithelial cells were studied at the electron-microscope level. Within 10 minutes after irradiation with 200 rads or more, the cell membrane showed morphological evidence of softening. Within 1 hour after administration of 3000 rads, alterations in the organization of mitochondrial cristae were found, and it was observed that such alterations became more general with passage of time until virtually all mitochondria were affected by days 4 and 5. Changes in nuclei, consisting in increase in size and in irregular contour, and the appearance of myeloid figures in the cytoplasm occurred with increasing frequency with increasing time interval after irradiation. Corn oil was absorbed and transported across the epithelium in about normal amounts by cells Which were on the villus at the time of irradiation. But cells appearing on the villus at 2 days and later showed a decrease in absorption and minimal transport across the epithelium. Cells from animals sacrificed 2 to 5 days after treatment showed a progressive depletion of ribonucleoprotein granules and a significant decrease in the number of mitochondria and membrane-bound vesicles. Possible interrelations of the observations are presented and discussed. (auth)
- Research Organization:
- Brookhaven National Lab., Upton, N.Y.; and Univ. of Texas, Galveston
- NSA Number:
- NSA-17-004420
- OSTI ID:
- 4771562
- Report Number(s):
- BNL-6077
- Journal Information:
- Radiation Res., Journal Name: Radiation Res. Vol. Vol: 17
- Country of Publication:
- United States
- Language:
- English
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