The distribution of calcium in the epiphyseal cartilage of the rat tibia measured with the electron probe X-ray microanalyzer

Journal Article · Wed Aug 01 00:00:00 EDT 1962 · Journal of Ultrastructure Research

DOI:https://doi.org/10.1016/S0022-5320(62)80026-4· OSTI ID:4768608

Brooks, E. J.; Tousimis, A. J.; Birks, L. S.

A description is given of the electron probe x-ray microanalyzer used to determine the Ca distribution in the epiphyseal plate of the rat tibia. Vacuum- coating of the speciMen with a thin layer of Al was added to the usual techniques for preparing thin section of biological specimens. Ca content was found to change from about 30 to 0% from the completely calcified cartilage edge toward the maturation zone of the epiphyseal plate. Variation in the concentration gradient may be related to the nonuniform distribution of hydroxyapattte crystals in the matrix collagen. (H.H.D.)

• Research Organization: Naval Research Lab., Washington, D.C.
• Sponsoring Organization: USDOE
• NSA Number: NSA-17-005727
• OSTI ID: 4768608
• Journal Information: Journal of Ultrastructure Research, Journal Name: Journal of Ultrastructure Research Journal Issue: 1-2 Vol. 7; ISSN 0022-5320
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

BIOLOGY AND MEDICINE
BONES
CALCIUM
CARTILAGE
COLLAGEN
DISTRIBUTION
ELECTRON MICROSCOPY
MEASURED VALUES
PROTEINS
RATS
TISSUES