Localization of Tc-99m MDP in epiphyseal growth plates of rats
The distribution and localization of Tc-99m methylene diphosphonate (Tc-MDP) in the epiphyseal growth plates of the rat were elucidated by contact and microautoradiography. The uptake of the tracer was found to be especially high in the calcified cartilage bars at the end of the vascular loops. In addition to areas of mineralization, increased uptake was found in the Howship's lacunae on the resorbing surfaces. This labeling corresponded with the fluorescence of tetracycline, which labeled both forming and resorbing surfaces, when given with short labeling interval. Distribution of Tc-MDP did not coincide with new production of collagen, as judged by H-3 proline labeling; nor was the uptake localized within cells with high alkaline phosphatase activity. The affinity of the tracer for the mineral phase was confirmed by decalcification of in vivo labeled sections with EDTA, whic showed loss of radioactivity in contrast to sections incubated in water. By chromatography the activity in the decalcification medium could not be distinguished from that of Tc-MDP.
- Research Organization:
- Rigshospitalet, Copenhagen, Denmark
- OSTI ID:
- 6806407
- Journal Information:
- J. Nucl. Med.; (United States), Journal Name: J. Nucl. Med.; (United States) Vol. 22:3; ISSN JNMEA
- Country of Publication:
- United States
- Language:
- English
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560172* -- Radiation Effects-- Nuclide Kinetics & Toxicology-- Animals-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AMINO ACIDS
ANIMAL TISSUES
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL LOCALIZATION
BIOSYNTHESIS
BODY
BONE SEEKERS
BONE TISSUES
CARBOXYLIC ACIDS
CHELATING AGENTS
COLLAGEN
CONNECTIVE TISSUE
EDTA
ESTERS
HOURS LIVING RADIOISOTOPES
INTERMEDIATE MASS NUCLEI
ISOMERIC NUCLEI
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
LABELLED COMPOUNDS
MAMMALS
METABOLISM
MICRORADIOGRAPHY
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANS
PHOSPHONIC ACID ESTERS
PHYSIOLOGY
PROTEINS
RADIOISOTOPES
RADIONUCLIDE KINETICS
RATS
RODENTS
SCLEROPROTEINS
SKELETON
SYNTHESIS
TECHNETIUM 99
TECHNETIUM ISOTOPES
TISSUES
TRACER TECHNIQUES
TRITIUM COMPOUNDS
UPTAKE
VERTEBRATES
YEARS LIVING RADIOISOTOPES