Factors influencing inactivation of infectivity and hemagglutinin of influenza virus by gamma radiation
The effects of various conditions on the production of vaccines from PR8 influenza A virus by irradiation in a Co 60 cell (at a rate of 1.25 Mrad/hr) are described. In purified virus suspensions in physiological saline, the hemagglutinin was destroyed more rapidly than infectivity. However, the addition of certain reagents reversed this effect. The most effective compounds for protection of the hemagglutinin during gamma irradiation are the sulfurcontaining amino acids (cystine, cysteine, and methionine) and compounds containing a ring structure such as histidine, tryptophan, tyrosine, phenylalanine, sodium p- aminohippurate (PAH), and sulfanilamide. Ascorbic acid, an antioxidant, also exerts a protective action. The effect of a given amount of gamma radiation on infectivity and hemagglutinin was similar regardless of whether the radiation was administered as a single dose or as 2 to 4 divided doses on different days. Experiments were repeated with other strains of influenza A and B with similar results. Since the protective reagents added to virus suspensions were not of greatly different molecular weight, it appears that their protective effect on the hemagglutinin during irradiation is more likely due to an interference with chemical action. This suggests that the formation of a strongly oxidizing medium during irradiation accounts for much of the destruction of both the hemagglutinin and the infectivity. With these agents it is therefore possible by means of gamma radiation to destroy the infectivity of an influenza virus suspension while retaining most of the antigenicity.
- Research Organization:
- Dept. of National Health and Welfare, Ottawa
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-17-015668
- OSTI ID:
- 4755788
- Journal Information:
- Canadian Journal of Microbiology, Vol. 7, Issue 4; Other Information: Orig. Receipt Date: 31-DEC-63; ISSN 0008-4166
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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Related Subjects
AMIDES
AMINES
AMINO ACIDS
ANTIBODIES
ANTIGENS
BIOCHEMISTRY
CHEMICAL REACTIONS
COBALT 60
CONFIGURATION
CYSTEINE
GAMMA RADIATION
GAMMA SOURCES
HEMAGGLUTININS
HIPPURIC ACID
HISTIDINE
IMMUNITY
IMPURITIES
INFECTIONS
INFLUENZA
MOLECULES
ORGANIC COMPOUNDS
ORGANIC SULFUR COMPOUNDS
OXIDATION
PHENYLALANINE
RADIATION DOSES
RADIATION EFFECTS
RADIATION PROTECTION
SODIUM CHLORIDES
SODIUM COMPOUNDS
SOLUTIONS
SULFONAMIDES
SUSPENSIONS
TRYPTOPHAN
TYROSINE
USES
VACCINES
VARIATIONS
VIRUSES
VITAMIN C
VITAMINS
WEIGHT