STUDIES OF THE RETICULOENDOTHELIAL SYSTEM (RES). I. MEASUREMENT OF THE PHAGOCYTIC CAPACITY OF THE RES IN MAN AND DOG

Journal Article · Fri Mar 01 04:00:00 EST 1963 · Journal of Clinical Investigation

DOI:https://doi.org/10.1172/JCI104729· OSTI ID:4749166

Iio, Masahiro; Wagner, Henry N.

When human serum albumin is aggregated under standardized conditions, a polymer is obtained that can be successfully used to study the phagocytic capacity of the reticuloendothelial system (RES) in man. In addition to careful control of pH, temperature, amount of agitation, and concentration of the albumin solution, it is necessary to monitor the changes in optical density, to insure adequacy of the prepared material by bioassay in man, or dog, or both, and to conduct simultameous control studies in normal subjects during the study of patients. Large doses of aggregated albumin can be safety administered over prolonged periods to human subjects without evidence of hypersensitivity or excessive radiation. The phagocytic capacity of the RES in both man and dog was determined experimentally with the analytical method of Michaelis and Menten. In man, this maximal rate of phagocytosis of aggregated albumin was 1.07 mg per minute per kg of body weight. (auth)

• Research Organization: Johns Hopkins Medical Institutions, Baltimore
• Sponsoring Organization: USDOE
• NSA Number: NSA-17-015571
• OSTI ID: 4749166
• Journal Information: Journal of Clinical Investigation, Journal Name: Journal of Clinical Investigation Journal Issue: 3 Vol. 42; ISSN 0021-9738
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ACIDITY
ALBUMINS
ANIMAL CELLS
BIOLOGY AND MEDICINE
BLOOD SERUM
BODY
DIFFUSION
DOGS
LIGHT
LYMPH SYSTEM
MAN
PHAGOCYTOSIS
POLYMERIZATION
QUANTITY RATIO
RADIATION DOSES
RETICULOENDOTHELIAL SYSTEM
SAFETY
SENSITIVITY
SOLUTIONS
TEMPERATURE
VARIATIONS
WEIGHT