THE INFLUENCE OF ROENTGEN RADIATION ON OXIDATIVE PHOSPHORYLATION IN ISOLATED MITOCHONDRIA

Journal Article · Sun Jan 01 04:00:00 EST 1961 · Acta Biologica et Medica Germanica (East Germany)

OSTI ID:4739555

Altenbrunn, H J; Kobbert, E

Mitochondria were sedimented from homogenates of rat liver in either isotonic sucrose medium or in isotonic KClmannitol medium. Oxidative phosphorylation, or the P/O ratio, was greater in sucrose than in the other medium in the presence of various substrates. Irradiation of mitochondria suspended in sucrose with 200-kv x rays at doses between 750 and 40000 r had no effect on respiration, phosphorylation, or P/O ratio in the presence of various substrates of the citric acid cycle, glutamic acid, or deg hydroxybutyric acid. In the presence of alpha -ketoglutarate, respiration and phosphorylation increased approximates 10 to 20% after 40000-r irradiation but the P/O ratio was unaffected. Irradiation of mitochondria suspended in isotonic KClmannitol solution resulted in a fall in respiration, phosphorylation, and the P/O ratio in the presence of all of the substrates tested. The metabolic activities of unirradiated mitochondria remained high when stored in isotonic sucrose for 24 hr but fell sharply by that time in the 2nd medium. (H.H.D.)

• Research Organization: Forschungsgemeinschaft der Deutschen Akademie der Wissenschaften, Berlin
• NSA Number: NSA-17-005784
• OSTI ID: 4739555
• Journal Information: Acta Biologica et Medica Germanica (East Germany), Journal Name: Acta Biologica et Medica Germanica (East Germany) Vol. Vol: 7; ISSN ABMGA
• Country of Publication: Country unknown/Code not available
• Language: German

Related Subjects

AMINO ACIDS
ANIMAL CELLS
BIOCHEMISTRY
BIOLOGY AND MEDICINE
CITRIC ACID
GLUTAMIC ACID
LIVER
METABOLISM
MITOCHONDRIA
ORGANIC ACIDS
OXIDATION
OXIDATIVE PHOSPHORYLATION
POTASSIUM CHLORIDES
RADIATION DOSES
RADIATION EFFECTS
RATS
SOLUTIONS
SUGARS
X RADIATION