OBSERVATIONS ON X-RAY-INDUCED RADICALS IN WHOLE BACTERIOPHAGE AND PHAGE NUCLEIC ACID

Journal Article · Fri Feb 01 04:00:00 EST 1963 · Intern. J. Radiation Biol.

DOI:https://doi.org/10.1080/09553006314550131· OSTI ID:4724242

Mueller, A

BS>Electron-spin-resonance (ESR) measurements on x-rayed purified bacteriophage of E. coli T2 and on DNA extracted from this phage yielded the following main results: The ESR absorption of DNA irradiated in vacuo at various temperatures showed a pronounced hyperfine structure if measured in vacuo at room temperature. When DNA is subjected to increasing doses of x rays in vacuo at room temperature saturation of radical concentration occurs at a few hundred kr. The initial energy expenditure measured at room temperature after irradiation at various temperatures ranges between 8 and 30 ev per radical induced. The ESR absorption of whole phage irradiated in vacuo at various temperatures is at room temperature equal to the absorption of phage-DNA plus that of a similar quantity of protein. The dependence of radical concentration on dose in whole phage irradiated in vacuo at room temperature is equal to the dose-dependence of DNA, plus that of a similar quantity of protein. (auth)

• Research Organization: Kernforschungszentrum, Karlsruhe, Ger.
• Sponsoring Organization: USDOE
• NSA Number: NSA-17-019845
• OSTI ID: 4724242
• Journal Information: Intern. J. Radiation Biol., Journal Name: Intern. J. Radiation Biol. Vol. Vol: 6
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

BACTERIA
BACTERIOPHAGES
BIOLOGY AND MEDICINE
ELECTRONS
ESCHERICHIA COLI
FREE RADICALS
MEASURED VALUES
NUCLEIC ACIDS
PROTEINS
RADIATION DOSES
RADIOCHEMISTRY
RESONANCE
SPIN
TEMPERATURE
X RADIATION