A SEMIAUTOMATIC DEVICE FOR MEASURING RADIOACTIVITY OF TWO-DIMENSIONAL PAPER CHROMATOGRAMS

Journal Article · Tue Jan 01 04:00:00 EST 1963 · Analytical Biochemistry (U.S.)

DOI:https://doi.org/10.1016/0003-2697(63)90053-8· OSTI ID:4724028

Moses, V; Lonberg-Holm, K K

A technique was developed for the automatic measurement of radioactivity on 2;dimensional paper chromatograms. The positions of radioactive compounds on the chromatograms are determined by radioautography and the appropriate areas are cut out from the chromatogram. The excised chromatogram spots are sandwiched between two continuous bands of thin plastic polyester film in an apparatus designed for this purpose. In another apparatus, the accumulated double band of polyester film bearing the chromatogram spots is automatically passed in a discontinuous manner between two closely opposed detector tubes. Each spot remains stationary between the detector tubes for a predetermined counting period. For each chromatogram spot the number of counts recorded, together with the elapsed time, is automatically printed onto paper tape. The double band of polyester film is then automatically advanced until the next spot lies between the detector tubes, and the cycle is repeated. (P.C.H.)

• Research Organization: Univ. of California, Berkeley
• NSA Number: NSA-17-010543
• OSTI ID: 4724028
• Report Number(s): UCRL-10112; 0003-2697
• Journal Information: Analytical Biochemistry (U.S.), Journal Name: Analytical Biochemistry (U.S.) Vol. Vol: 5; ISSN ANBCA
• Country of Publication: United States
• Language: English

Related Subjects

CHEMISTRY
CHROMATOGRAPHY
COUNTERS
MEASURED VALUES
PAPER
PHOTOGRAPHIC FILM DETECTORS
POLYESTERS
QUANTITATIVE ANALYSIS
RADIATION DETECTORS
RADIOACTIVITY
RADIOAUTOGRAPHY
RECORDING SYSTEMS