LIQUID SCINTILLATION COUNTING OF C$sup 14$ PAPER CHROMATOGRAMS
A method is described for the counting of C/sup 14/ biological materials on paper chromatograms. Paper sections are placed in counting vials and are extracted by the solubilizing agent, hyamine. Scintillation solvent is then added, and the samples are counted by a liquid scintillation spectrometer. Absolute radioactivity of each spot can be obtained by any of the following three correction methods. One method involves the dilution of the sample by scintillator solvent, the other uses an internal standard, and the last method involves correction of the observed radioactivity by the counting efficiency obtained from the ratio of counts in two separate windows of the spectrometer. Any of the three methods will give quantitative data if the extraction of the sample from the paper by a solubilizing agent is over 50%. The methods have been applied to the counting of various C/sup 14/ amino acids and C/sup 14/ sugars on paper sections with excellent recovery in radioactivity and reproducibility. (auth)
- Research Organization:
- Tokyo Univ.
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-16-000136
- OSTI ID:
- 4823464
- Journal Information:
- Analytical Biochemistry (U.S.), Journal Name: Analytical Biochemistry (U.S.) Vol. Vol: 2; ISSN ANBCA
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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