Effects of low concentrations of benzene on mouse hematopoietic cells in vivo: A preliminary report
- Chemical Industry Institute of Toxicology, Research Triangle Park, NC (United States)
Evaluation of benzene-induced hematotoxicity following exposure to low concentrations is important for understanding mechanisms of toxicity and determining the dose response at benzene levels close to the current occupational exposure limit 0 ppm. Male B6C3F1 mice were exposed to 0, 1, 10, 100, or 200 ppm benzene by inhalation for 6 hr/day, 5 days/week for 1, 2, 4, or 8 weeks. At each sampling time, we evaluated primitive and committed progenitor cells, differentiating and maturing lineage-specific cells, and stromal cells in the bone marrow; T and B lymphocytes of the spleen and thymus; micronucleated reticulocytes and erythrocytes; and standard blood parameters. At 100 and 200 ppm benzene, there were rapid and significant reductions in number of reticulocytes in the blood, B lymphocytes in the bone marrow and spleen, and an increased frequency of micronucleated reticulocytes in the bone marrow. At 10 ppm, the only parameter affected was a transient reduction in the number of splenic B lymphocytes. There were no significant effects induced by 1 ppm benzene in this study. The present study suggests numbers of B lymphocytes and maturing erythrocytes, and frequency of micronucleated reticulocytes are sensitive indicators of benzene-induced hematotoxicity and will be useful in further investigation of the hematotoxicity induced by 10 to 100 ppm benzene. 7 refs., 2 figs.
- Sponsoring Organization:
- USDOE
- OSTI ID:
- 472172
- Report Number(s):
- CONF-9506288-; ISSN 0091-6765; TRN: 97:001626-0024
- Journal Information:
- Environmental Health Perspectives, Vol. 104, Issue Suppl.6; Conference: Benzene `95: international conference on benzene toxicity, carcinogenesis, and epidemiology, Piscataway, NJ (United States), 17-20 Jun 1995; Other Information: PBD: Dec 1996
- Country of Publication:
- United States
- Language:
- English
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