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THE USE OF STANDARD SLIDES IN SEMI-QUANTITATIVE RADIOAUTOGRAPHY WITH TRITIATED COMPOUNDS

Journal Article · · Stain Technology (U.S.)
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By use of the standard-slide'' method it is possible to determine if different batches of radioautographs, or even different radioautographs in the same batch, have been processed in an identical manner, and if not, what corrections are necessary to obtain a valid comparison between different radioautographs. With the technique described, essentially identical smears can be prepared from a pooled suspension of Ehrlich ascites tumor cells labeled with tritiated thymidine. In these smears, the percentage of labeled cells and the mean grain count per labeled cell vary within marrow limits; in a given slide, within 2 to 5% of the mean of the slide. In smears processed together in the same manner, the percentage of labeled ceils (calculated on 1000 cells) varies within 2%, and the mean grain count per labeled cell (calculated on 50 cells) between 15 and 20% of the mean of all smears. Once the limits of variability are known, smears from the same cell population can be used to ensure the accuracy of procedures performed on separate batches of radioautographs that have been processed separateiy but in a presumably identical manner. A comparison of the mean grain counts of standard smears from separate batches indicates if variations have occurred during the radioautographic procedure. Any tritiated compound may be used to prepare standard slides. (H.H.D.)
Research Organization:
Northwestern Univ., Chicago
Sponsoring Organization:
USDOE
NSA Number:
NSA-17-027010
OSTI ID:
4717209
Journal Information:
Stain Technology (U.S.), Journal Name: Stain Technology (U.S.) Vol. Vol: 38; ISSN STTEA
Country of Publication:
Country unknown/Code not available
Language:
English

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Related Subjects

ANIMAL CELLS
BIOLOGY AND MEDICINE
EHRLICH ASCITES
LABELLED COMPOUNDS
RADIOAUTOGRAPHY
TRACER TECHNIQUES
TRITIUM
TUMORS