EFFECTS OF HISTOLOGIC AND HISTOCHEMICAL PROCEDURES ON THE INTENSITY OF THE LABEL IN RADIOAUTOGRAPHS OF CELLS LABELED WITH TRITIATED COMPOUNDS

Journal Article · Tue Oct 01 04:00:00 EDT 1963 · Laboratory Investigation (U.S.)

OSTI ID:4103645

Baserga, R; Kisielski, W E

Ehrlich ascites tumor cells were labeled in vivo with one of three tritiated compounds (thymidine, cytidine, or leucine) and, after 30 minutes, were examined by radioautography. Both Carnoy's solution and absolute methanol proved to be reliable fixatives, removing from cells equal amounts of radioactivity. The fixative-soluble radioactivity presumably represents the fraction of precursor that has not been incorporated into nucleic acids or proteins. Treatment with ribonuclease or cold perchloric acid removed all radioactivity from cells labeled with tritiated cytidine, a precursor of ribonucleic acid, but not from cells labeled with tritiated thymidine. Treatment with deoxyribonuclease removed radioactivity incorporated into deoxyribonucleic acid, but it also affected to a lesser extent the ribonucleic acid label. A comparison of sections and smears from the same sample indicated that, in smears of cells labeled with tritiated leucine, some of the silver grains in the radioautographic emulsion overlying the nucleus are due to radioactivity incorporated into the thin layer of cytoplasm interposed between the nucleus and the emulsion. (auth)

• Research Organization: Northwestern Univ., Chicago and Argonne National Lab., Ill.
• NSA Number: NSA-18-008056
• OSTI ID: 4103645
• Journal Information: Laboratory Investigation (U.S.), Journal Name: Laboratory Investigation (U.S.) Vol. Vol: 12; ISSN LAINA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AMINO ACIDS
BIOCHEMISTRY
BIOLOGY AND MEDICINE
CANCER
CYTIDINE
CYTOLOGY
DISTRIBUTION
DNA
EHRLICH ASCITES
EMULSIONS
ENZYMES
LEUCINE
METHANOL
NUCLEIC ACIDS
PERCHLORIC ACID
PROTEINS
RADIOACTIVITY
RADIOAUTOGRAPHY
RIBONUCLEIC ACID
SILVER
SOLUBILITY
SOLUTIONS
THYMIDINE
TRACER TECHNIQUES
TRITIUM
VARIATIONS