A simple procedure for the destaining of stripping-film autoradiographs
Journal Article
·
· Journal of Pathology and Bacteriology (Scotland)
Trypsin digestion of stripping-film radioautographs after photographic processing and staining removes the excess gelatin without affecting the intensity of the radioautograph, and results in a thin colorless emulsion that makes microscopic observation and photography easier. Methanol-fixed smears or Carnoy-fixed tissue sections, labeled with tritiated thymidine and unstained, were radioautographed, and the radioautographs were developed, fixed, stained with hematoxylin, and counterstained with eosin. The slides were air-dried, rehydrated for 10 min in distilled water, and incubated in a 0.01% solution of trypsin at 37 °C for 6-8 min. In stripping-film radioautographs digested as described, the 10 mu supporting layer of gelatin is completely removed, but the sensitized layer containing the developed silver grains is not affected.
- Research Organization:
- Northwestern Univ., Chicago
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-17-026988
- OSTI ID:
- 4663253
- Journal Information:
- Journal of Pathology and Bacteriology (Scotland), Journal Name: Journal of Pathology and Bacteriology (Scotland) Journal Issue: 1 Vol. 84; ISSN 0368-3494
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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