IN VIVO CHANGES IN UROCANIC ACID OF GUINEA PIG AND HUMAN EPIDERMIS BY ULTRAVIOLET LIGHT
Control skin and skin irradiated with ultraviolet light (UV) were examined for chemical differences resulting from the UV irradiation. It was found that acid extracts of skin exposed to three minimal erythema dose of UV from a hot quartz mercury vapor lamp possess a marked decrease in the specific UV absorbency in the 260 m mu portion of the spectrum. It is shown that the decrease in specific UV absorbency is due largely to decreased amounts of urocanic acid in the extract. It is further shown that almost half of the urocanic acid isolated after irradiation is the cis isomer. Only the trans modification was found in normal, non-irradiated extracts. A method for separation of the cis and trans isomers of urocanic acid, using ion exchange methods, is outlined. The infrared absorption spectra of cis and trans urocanic acid isolated from skin is presented with similar spectra for the authentic trans isomer and in vitro prepared cis urocanic acid. The cis isomer of urocanic acid from UV irradiated skin was characterized by melting point, mobility in paper chromatographic systems, color formation with diazotized aromatic amines, and UV absorption spectra characteristics. Infrared absorption data support present assignment of cis and trans configurations in these substances. (Dissertation Abstr. 23: May 1963)
- Research Organization:
- Originating Research Org. not identified
- NSA Number:
- NSA-17-028767
- OSTI ID:
- 4694856
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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Related Subjects
ACIDS
ACRYLIC ACID
AMINES
BIOLOGY AND MEDICINE
CHROMATOGRAPHY
GUINEA PIGS
IMIDAZOLES
INFRARED RADIATION
ION EXCHANGE
ISOMERS
MAN
MELTING POINTS
MERCURY
ORGANIC ACIDS
QUALITATIVE ANALYSIS
QUANTITY RATIO
QUARTZ
RADIATION DOSES
RADIATION EFFECTS
RADIATION SOURCES
SKIN
SOLVENT EXTRACTION
SPECTRA
ULTRAVIOLET RADIATION
UROCANIC ACID
VAPORS