ON THE MECHANISM OF THE THERAPEUTIC ACTION OF HIGHLY POLYMERIZED DNA IN RADIATION SICKNESS

Journal Article · Mon Dec 31 23:00:00 EST 1962 · Radiobiologiya (U.S.S.R.) For English translation see Radiobiology (Engl. Transl.)

OSTI ID:4694051

Libinzon, R E; Konstantinova, V V; Popova, T G; Rogacheva, S A

To clarify the mechanism of action of highly polymerized DNA in acute radiation sickness, the fate of administered compounds in the normal and irradiated animals was studied directly, together with the effect of DNA on proliferation processes in bone marrow of irradiated animals. Fifty-five rats (there were 25 controls) were irradiated with 1000-r doses from Co/sup 60/, injected intraabdominally 24 hr later with 5 to 6 mg of highly polymerized DNA as liver, spleen, or thymus preparation in physiological saline, and 6 to 72 hr later were sacrificed. Homogenates of tissues were separated into DNA, RNA, and acid-soluble fractions. The DNA preparations, marked with P/sup 32/, were obtained from young rats partialiy hepatectomized and given NaHP/sup 32/O/sub 4/. The labeled DNA specific activity was highest in the liver in the first 6 hr after administration, 115% in the controls and 173% in the irradiated; it fell to 6% in both at 24 hr, and then rose at 72 hr to 70 and 30%, respectively. Spleen and bone marrow curves were somewhat similar in shape. The highest DNA activity was in the bone marrow in normal animals and in the liver in irradiated ones, and in most cases was lower in the irradiated than in the controls. In the bone marrow of the treated animals the mitotic activity was higher than in those not treated, and there were more nucleated cells. (TTT)

• Research Organization: Originating Research Org. not identified
• NSA Number: NSA-17-031973
• OSTI ID: 4694051
• Journal Information: Radiobiologiya (U.S.S.R.) For English translation see Radiobiology (Engl. Transl.), Journal Name: Radiobiologiya (U.S.S.R.) For English translation see Radiobiology (Engl. Transl.) Vol. Vol: 3; ISSN RADOA
• Country of Publication: Country unknown/Code not available
• Language: Russian

Related Subjects

ACIDS
AGE
BIOLOGY AND MEDICINE
BONE MARROW
COBALT 60
CYTOLOGY
DIAGRAMS
DISTRIBUTION
DNA
GAMMA SOURCES
IRRADIATION
LABELLED COMPOUNDS
LIVER
MEDICINE
METABOLISM
MITOSIS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
PHOSPHORUS 32
QUANTITY RATIO
RADIATION DOSES
RADIATION SICKNESS
RATS
REGENERATION
RIBONUCLEIC ACID
SODIUM CHLORIDES
SODIUM PHOSPHATES
SOLUBILITY
SOLUTIONS
SPLEEN
THERAPY
THYMUS
TRACER TECHNIQUES
VARIATIONS