Autoradiography of Tritium-Containing Thin Layer Chromatograms

Journal Article · Thu Oct 31 23:00:00 EST 1963 · Analytical Chemistry

DOI:https://doi.org/10.1021/ac60205a071· OSTI ID:4691928

Sheppard, Herbert.; Tsien, W. H.

A simple and sensitive method of preparing autoradiograms from thin layer chromatograms containing tritiumlabeled substances was developed. The chromatograms are exposed to photographic plates prepared with nuclear track emulsion. Using this method, as little as 0.01 mu C of H³ is detectable in a compact spot. (P.C.H.)

• Research Organization: CIBA Pharmaceutical Co., Summit, N.J.
• Sponsoring Organization: USDOE
• NSA Number: NSA-17-040732
• OSTI ID: 4691928
• Journal Information: Analytical Chemistry, Journal Name: Analytical Chemistry Journal Issue: 12 Vol. 35; ISSN 0003-2700
• Publisher: American Chemical Society (ACS)
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

CHEMISTRY
CHROMATOGRAPHY
LABELLED COMPOUNDS
LAYERS
NUCLEAR EMULSIONS
PARTICLE TRACKS
PHOTOGRAPHIC FILM
RADIOAUTOGRAPHY
THICKNESS
TRACE AMOUNTS
TRITIUM