AUTORADIOGRAPHY OF TRITIUM-LABELLED COMPOUNDS ON PAPER CHROMATOGRAMS
Chromatograms of titrated progesterone of known activity were prepared on Whatman No. 1 paper treated with the Bush A solvent system. After drying, the strips were taken into a dark-room and passed through a trough of melted photographic emulsion at 50 deg C. Ilford K2 emulsion diluted by an equal volume of distilled water was used. The strips were hung at room temperature for 2 hours to dry and then placed in a light-proof box with about 20 gm of dried silica gel and left in a cold room at 5 deg C for periods ranging from one to four weeks. They were then processed in tanks of standard x-ray developer for 5 to 6 min, 10 to 15 min in fixer, washed for 2 hr in running water, and dried. The technique is reported to be very sensitive and applicable to the study of tritium-labeled compounds. (C.H.)
- Research Organization:
- Univ. of Birmingham, Eng.
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-14-001473
- OSTI ID:
- 4238616
- Journal Information:
- Nature, Journal Name: Nature Vol. Vol: 184, Suppl. No. 10
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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