cDNA cloning, tissue distribution, and chromosomal localization of myelodysplasia/Myeloid Leukemia Factor 2 (MLF2)

Journal Article · Mon Jul 15 04:00:00 EDT 1996 · Genomics

DOI:https://doi.org/10.1006/geno.1996.0376· OSTI ID:469051

Kuefer, M U; Valentine, V; Behm, F G ^[1]

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1. St. Jude Children`s Research Hospital, Memphis, TN (United States); and others

A fusion gene between nucleophosmin (NPM) and myelodysplasia/myeloid leukemia factor 1 (MLF1) and myelodysplasia/myeloid leukemia factor 1 (MLF1) is formed by a recurrent t(3;5)(q25.1;q34) in myelodysplastic syndrome and acute myeloid leukemia. Here we report the identification of a novel gene, MLF2, which contains an open reading frame of 744 bp encoding a 248-amino-acid protein highly related to the previously identified MLF1 protein (63% similarity, 40% identity). In contrast to the tissue-restricted expression pattern of MLF1, and MLF2 messenger RNA is expressed ubiquitously. The MLF2 gene locus was mapped by fluorescence in situ hybridization to human chromosome 12p13, a chromosomal region frequently involved in translocations and deletions in acute leukemias of lymphoid or myeloid lineage. In a physical map of chromosome 12, MLF2 was found to reside on the yeast artificial chromosome clone 765b9. Southern blotting analysis of malignant cell DNAs prepared from a series of acute lymphoblastic leukemia cases with translocations involving chromosome arm 12p, as well as a group of acute myeloid leukemias with various cytogenetic abnormalities, failed to reveal MLF2 gene rearrangements. 19 refs., 2 figs.

• OSTI ID: 469051
• Journal Information: Genomics, Journal Name: Genomics Journal Issue: 2 Vol. 35; ISSN 0888-7543; ISSN GNMCEP
• Country of Publication: United States
• Language: English

Related Subjects

55 BIOLOGY AND MEDICINE
BASIC STUDIES
CHROMOSOMAL ABERRATIONS
DNA HYBRIDIZATION
DNA SEQUENCING
DNA-CLONING
ETIOLOGY
FLUORESCENCE
GENE MUTATIONS
GENE REGULATION
GENES
GENETIC MAPPING
HEREDITARY DISEASES
HUMAN CHROMOSOME 12
HUMAN CHROMOSOME 3
HUMAN CHROMOSOME 5
MYELOID LEUKEMIA
PATHOGENESIS
POLYMERASE CHAIN REACTION
STRUCTURE-ACTIVITY RELATIONSHIPS
TISSUE DISTRIBUTION
TUMOR CELLS