MORPHOLOGICAL AND CYTOLOGICAL STUDY OF HUMAN AMNION CELLS IN THE COURSE OF RECOVERY FROM RADIATION INJURY

Journal Article · Sun Jan 01 04:00:00 EST 1961 · Texas Rept. Biol. Med.

OSTI ID:4686242

Mizutani, M; Nakanishi, Yuh H; Pomerat, C M

Human amnion cells (Fernandes strain) cultured in vitro were irradiated with 1000-r doses of Co/sup 60/ gamma rays and examined by staining techniques for the next 5 days, The number of cells in the control cultures increased until a plateau was reached at 4 days, then began to decrease, whereas irradiated cultures showed a progressive decrease in cell numbers during the 5-day period. Mitotic index in nonirradiated cultures reached a max on the first day, then showed a gradual decrease. In the irradiated cultures, the mitotic activity of the cells was remarkably inhibited on the first day, suddenly increased on the second day, and reached max on the third day, after which the mitotic index fell rapidly. The later higher values may result from the accumulation of the cells at metaphasic stage. In most metaphasic figures the chromosomes showed abnormalities. These severe chromosomal aberrations might inhibit migration of chromosomes to the poles at anaphase, so that metaphasic cells underwent degeneration in this condition without advancing further. Control cultures showed a constant value of giant cells at 5 days, and irradiated cultures reached a max on the third day after irradiation. The incidence of giant cells on the second day was higher in the irradiated cultures than in the controls. The number of multinucleate cells, consisting of both giant and nongiant elements, significantly increased in irradiated cultures, as compared with that in control cultures. A gradual shift in the modal value of chromosomes toward lower numbers with successive passages of cultures is suggestive of selective adaptation. (H.H.D.)

• Research Organization: Univ. of Texas, Galveston
• NSA Number: NSA-17-028766
• OSTI ID: 4686242
• Journal Information: Texas Rept. Biol. Med., Journal Name: Texas Rept. Biol. Med. Vol. Vol: 19
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AMNION CELLS
ANIMAL CELLS
BIOLOGY AND MEDICINE
CHROMOSOMES
COBALT 60
CYTOLOGY
GAMMA RADIATION
IRRADIATION
MAN
MITOSIS
MUTATIONS
QUANTITY RATIO
RADIATION DOSES
RADIATION EFFECTS
RADIATION INJURIES
RECOVERY
TISSUES