Effect of X-Ray on Antigen Metabolism in the Rabbit

Journal Article · Sat Jun 01 04:00:00 EDT 1963 · Radiation Research

DOI:https://doi.org/10.2307/3571458· OSTI ID:4661261

Rubinstein, H. M.; Perikh, H.

Bovine serum albumin conjugated to S³⁵-sulfanilic acid was injected intravenously in standard doses into three groups of rabbits: Group A (control): normal rabbits; group B: rabbits injected 18 hours after 450 r of wholebody irradiation (primary antibody response inhibited); and group C: rabbits injected immediately after 450 r of whole-body irradiation (primary antibody response not inhibited). The plasma, liver, spleen, lymph node, kidney, appendix, and lung were studied for radioactivity and protein content at 1, 7, and 21 days after injection. In all groups, the retention of radioactivity in the tissues decreased with time. The overall metabolism of the antigen by lymph node was retarded when compared to other tissues. There were no differences among all three groups in total-organ retention of antigen at all time intervals. When group A was compared to groups B and C, there were certain differences when antigen retention was expressed in terms of tissue weight or protein content. These differences were attributed to organ-weight changes secondary to irradiation. These differences did not appear when group B was compared to group C. The cells responsible for the retention and degradation of the bulk of injected antigen are not inhibited by irradiation under conditions which inhibit the primary antibody response. When large amounts of antigen are injected, only a small fraction may be important in induction of antibody formation.

• Research Organization: Michael Reese Hospital, Chicago; Chicago Medical School
• Sponsoring Organization: USDOE
• NSA Number: NSA-17-027178
• OSTI ID: 4661261
• Journal Information: Radiation Research, Journal Name: Radiation Research Journal Issue: 2 Vol. 19; ISSN 0033-7587
• Publisher: Radiation Research Society
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ALBUMINS
ANTIBODIES
APPENDIX
BLOOD PLASMA
BLOOD SERUM
BODY
INTESTINE
KIDNEYS
LABELLED COMPOUNDS
LIVER
LYMPH NODES
LYMPH SYSTEM
METABOLISM
PROTEINS
QUANTITATIVE ANALYSIS
QUANTITY RATIO
RABBITS
RADIATION DOSES
RADIOACTIVITY
SPLEEN
SULFANILIC ACID
SULFONIC ACIDS
SULFUR 35
X RADIATION