TRANSFER OF RABBIT LYMPH NODE CELLS TO NEONATAL RECIPIENT RABBITS

Journal Article · Thu Feb 01 04:00:00 EST 1962 · Journal of Immunology (U.S.)

OSTI ID:4839362

Harris, T N; Harris, S; Farber, M B

Previous experiments were continued by investigating the immunologic responsiveness of newborn rabbits to homologous spleen cells and the effect of a prior injection of other homologous cells. When lymph node cells were obtained from adult rabbits, incubated in vitro with a filtrate of trypsin-treated Shigella paradysenteriae, and transferred to irradiated neonatal recipient rabbits, agglutinins to Shigella appeared subsequently in the serums of these recipients. When approximately the same number of cells were given to neonatal and to adult recipients, the amount of antibody produced per transferred cell was estimated to be about 1/5 as much in the neonatal as the adult recipient. However, with decreasing numbers of cells transferred to the neonatal rabbits, there was an increase in the highest relative values of antibody produced per cell until, when the number of cells were approximately proportional to the weights of the recipients, the maximum value for the amount of antibody per transferred cell in neonatal recipients was about 1/2 of the level in 1-kg recipients. X irradiation of the neonatal recipients prior to transfer led to higher antibody levels than those found in nonirradiated litter-mates, in the same range of ratios as that found in older recipients. Cells obtained from lymph node draining sites of injection of heterologous anitgens did not lead to significantly different antibody titers than in the case of cells from uninjected donor rabbits. Neonatal recipient rabbits previously injected with rabbit leukocytes, even on the first day of life, yielded lower agglutinin titers after lymph node cell transfer carried out one week later. Complete suppression of the antibody-inducing effect of transferred lymph node cells was found after the injection of 10/sup 7/leukocytes, and decreasing degrees of suppression in the case of 10/sup 6/ and 10/sup 5/ leukocytes, respectively. Thus, the day-old rabbit can respond immunologically to leukocytes and requires approximately the same number of cells for antigenic stimulation as older rabbits. (H.H.D.)

• Research Organization: Univ. of Pennsylvania, Philadelphia
• NSA Number: NSA-16-020275
• OSTI ID: 4839362
• Journal Information: Journal of Immunology (U.S.), Journal Name: Journal of Immunology (U.S.) Vol. Vol: 88; ISSN JOIMA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AGE
ANIMAL CELLS
ANTIBODIES
ANTIGENS
BACTERIA
BIOLOGY AND MEDICINE
IMMUNITY
LEUCOCYTES
LYMPH NODES
LYMPH SYSTEM
QUANTITY RATIO
RABBITS
RADIATION EFFECTS
SPLEEN
TRANSPLANTS
X RADIATION