QUANTITATIVE STUDIES CONCERNING THE RADIATION PROTECTIVE EFFECT OF CELL- FREE MOUSE AND GUINEA PIG SPLEEN EXTRACTS
Journal Article
·
· Arch. Intern. Pharmacodyn.
OSTI ID:4659479
S>Cell-free saline extracts of lyophilized spleen were injected in sublethally and lethally irradiated animals. Mouse spleen extract protects both mice and guinea pigs and vice versa. This suggests a common factor as the protective principle of these extracts; but, the protective effect is not parallel with the total protein content. Though mouse spleen extracts showed 50% less total protein content than guinea pig spleen extracts, they effectively reduced mortality of guinea pigs at various radiation doses. A spleen equivalent of 346 to 505 mg of frozen wet wt per injection was protective. In contrast, the spleen equivalent of guinea pig extracts necessary for the protection of both species varied between 970 and 1870 mg per injection. Thus, the guinea pig spleen which is 4 to 5 times heavier than mouse spleen contains less protective factor per unit wt. There seems to exist a threshold value for the spleen equivalent for each species of donor animals below which a reduction of radiation mortality cannot be effected. The irradiation mortality rate influences the amount of spleen equivalent to extracts necessary for protection. Preliminary paper chromatographic studies of extracts point to the significance of relative proportions of amino acids. Hence, free amino acids may represent the effective principle in these extracts, but other chemical entitles also must be considered. (TCO)
- Research Organization:
- Naval Medical Research Inst., Bethesda, Md.
- NSA Number:
- NSA-17-033758
- OSTI ID:
- 4659479
- Journal Information:
- Arch. Intern. Pharmacodyn., Journal Name: Arch. Intern. Pharmacodyn. Vol. Vol: 132
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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