Effect of Some Metabolites and Antimetabolites on the Breakdown of Deoxyribonucleic Acid and on the Colony-Forming Ability of Escherichia coli B after Gamma Irradiation. II. Effect of Phosphorylated Derivatives of Adenosine and Pyrophosphate

Journal Article · Mon Jul 01 04:00:00 EDT 1963 · Radiation Research

DOI:https://doi.org/10.2307/3571465· OSTI ID:4653689

Kos, E.; Drakulić, M.; Drakulic, M.

A complete inhibition of the biosynthesis of DNA in unirradiated E. coli B as well as a total inhibition of its breakdown in gamma -irradiated bacteria was observed if the cells were incubated in acetate buffer in the presence of ATP. In the presence of ADP or of AMP-3- and AMP-5', the breakdown of DNA in the irradiated cells is only partially inhibited. If inorganic pyrophosphate is added to the acetate buffer, both the biosynthesis of DNA in the controls and its breakdown in the irradiated suspension are somewhat accelerated. The effects of the above compounds on the colony-forming ability of E. coli B were also studied. The results of these experiments are compared with those obtained in the previous work, and their meaning is discussed.

• Research Organization: Institute "Ruder Boskovic," Zagreb; Univ. of Zagreb
• Sponsoring Organization: USDOE
• NSA Number: NSA-17-033727
• OSTI ID: 4653689
• Journal Information: Radiation Research, Journal Name: Radiation Research Journal Issue: 3 Vol. 19; ISSN 0033-7587
• Publisher: Radiation Research Society
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
ACETATES
ADENOSINE
AMINES
ATP
BACTERIA
BIOCHEMISTRY
CHEMICAL REACTIONS
DNA
ENZYMES
ESCHERICHIA COLI
GAMMA RADIATION
METABOLISM
NUCLEIC ACIDS
ORGANIC NITROGEN COMPOUNDS
PHOSPHATES
PYROPHOSPHATES
RIBONUCLEIC ACID