Interaction of manganous ions with DNA repair after uv irradiation in Proteus mirabilis

Journal Article · Sun Dec 31 23:00:00 EST 1972 · Stud. Biophys., v. 36-37, pp. 263-269

OSTI ID:4421655

Hofemeister, J

From international conference on the bases of the biological effects of ultraviolet radiation; Brno, Czechoslovakia (2 Oct Following uv irradiation cells were incubated in a Tris-HCl buffer supplemented with glucose, MnCla, or MgCl/sub 2/. Following incubation in LHR buffer, cells were plated on a medium containing caffeine; there was a low sensitivity of the unirradiated wild-type cells under these conditions. However, after uv irradiation a nearly complete inhibition of recovery was observed. The known inhibitory action of caffeine and 2,4- dinitrophenol was compared with that of Mn lons on liquid-holding recovery, dimer excision, and breakdown of DNA after uv irradiation. Liquid-holding recovery was inhibited by caffeine and Mn ions, whereas 2,4-dinitrophenol had a relatively weak influence on liquid-holding recovery. Mn ions and 2,4-dinitrophenol lowered the dimer excision rate to about 70% of the MgCl/sub 2/-containing control. Caffeine inhibited the breakdown of DNA after uv irradiation while Mn ions stimulated DNA breakdown. (HLW)

• Research Organization: Zentralinstitut fuer Genetik und Kulturpflanzenforschung, Berlin
• NSA Number: NSA-29-000571
• OSTI ID: 4421655
• Journal Information: Stud. Biophys., v. 36-37, pp. 263-269, Journal Name: Stud. Biophys., v. 36-37, pp. 263-269; ISSN STBIB
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

*CAFFEINE-- RADIOSENSITIVITY EFFECTS
*DINITROPHENOL-- RADIOSENSITIVITY EFFECTS
*DNA-- BIOLOGICAL REPAIR
*MANGANESE COMPOUNDS-- RADIOSENSITIVITY EFFECTS
*PROTEUS-- BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
CHEMICAL RADIATION EFFECTS
DECOMPOSITION
DIMERS
INHIBITION
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
PHOTON BEAMS
RESPONSE MODIFYING FACTORS
ULTRAVIOLET RADIATION