Species variations in binding of $sup 63$Ni(II) by serum albumin

Journal Article · Thu Mar 01 04:00:00 EST 1973 · Res. Commun. Chem. Pathol. Pharmacol., v. 5, no. 2, pp. 459-472

OSTI ID:4418974

Callan, W M; Sunderman, Jr, F W

Equilibrium dialysis was employed in order to compare the in vitro binding of /sup 63/Ni(II) by purified serum albumin from five mammalian species. These measurements showed that the /sup 63/Ni(II) affinities of canine and porcine serum albumins are substantially less than the affinities of human, rabbit, and rat serum albumins. Thus, when 0.05 mM solutions of albumins from the five species were dialyzed at pH 7.4 against an equimolar solution of /sup 63/ NiCl/sub 2/, 87 to 89% of total /sup 63/Ni(II) became bound to human, rabbit, and rat serum albumins, compared to values of 77% and 55% of total /sup 63/Ni(II), which became bound to porcine and dog albumins, respectively. These species variations in the affinities of albumin for /sup 63/Ni(II) are responsible, at least in part, for species differences in the proportions of serum ultrafiltrable and protein-bound nickel. (auth)

• Research Organization: Univ. of Connecticut, Farmington
• NSA Number: NSA-29-000883
• OSTI ID: 4418974
• Report Number(s): COO--3140-15
• Journal Information: Res. Commun. Chem. Pathol. Pharmacol., v. 5, no. 2, pp. 459-472, Journal Name: Res. Commun. Chem. Pathol. Pharmacol., v. 5, no. 2, pp. 459-472; ISSN RCOCB
• Country of Publication: United States
• Language: English

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