Contribution to the study of the mechanism of labelling human serum albumin (HSA) with technetium-99m

Journal Article · Wed Oct 31 23:00:00 EST 1973 · Int. J. Appl. Radiat. Isotop., v. 24, no. 11, pp. 621-626

DOI:https://doi.org/10.1016/0020-708X(73)90088-4· OSTI ID:4388201

Zolle, I; Oniciu, L; Hoefer, R

The labelling reaction using ferric chloride and ascorbic acid with the / sup 99m/Tc eluate was investigated. Ion exchange chrom tography was used to separate the reaction components at acidic and alkaline pH. /sup 99m/Tc was identified as an anion which was not complexed with ascorbic acid. Coprecipitation was used to differentiate between pertechnetate and a reduced form of technetium. Only the reduced technetium showed coprecipitation with ferrous/ ferric hydroxide. Coprecipitation was favored at elevated pH, suggesting that a hydrated /sup 99m/Tc oxide was formed. It is believed that this labels the albumin. (auth)

• Research Organization: Univ., Vienna
• NSA Number: NSA-29-007611
• OSTI ID: 4388201
• Journal Information: Int. J. Appl. Radiat. Isotop., v. 24, no. 11, pp. 621-626, Journal Name: Int. J. Appl. Radiat. Isotop., v. 24, no. 11, pp. 621-626; ISSN IJARA
• Country of Publication: United Kingdom
• Language: English

Related Subjects

*ALBUMINS-- LABELLING
*TECHNETIUM 99-- RADIOCHEMISTRY
ANIONS
ASCORBIC ACID
BLOOD SERUM
CHEMICAL REACTION KINETICS
COPRECIPITATION
HYDRATION
ION EXCHANGE CHROMATOGRAPHY
IRON CHLORIDES
IRON HYDROXIDES
ISOMERIC NUCLEI
MAN
N40420* --Chemistry--Radiochemistry & Nuclear Chemistry-- Properties of Radioactive Materials
PERTECHNETATES
PH VALUE
REDUCTION
TECHNETIUM OXIDES