Inactivation of mycoplasma in seed virus stocks using gamma radiation
A method was developed for the elimination of viable Mycoplasma in reference seed virus stocks. It was found that various species of Mycoplasma (such as M. pneumoniae, M. arthritidis, M. hominis, M. Salivarium, M. orale types I and II, M. meleagridis, and several unidentified species isolated from tissue cultures) were inactivated more rapidly by gamma radiation than all viruses tested. By the use of selected radiation doses, high concentrations of Mycoplasma species could be inactivated in virus suspensions of polioviruses types I and III, coxsackie viruses types A-7, A-9, B-3, and B-6, echoviruses types 1, 9, 12, and 20, herpes simplex, rubella, measles, and adenovirus type 7a, without inactivating all viable virus. After irradiation, the remaining viable virus could be propagnted as well as the original strain and showed no change in reactivity with homologous or heterologous antisera. After storage for two months at --70 deg C, the irradiated virus showed no decrease either in viability or in specific reactivity. By this method, reference seed virus stocks could be prepared free of viable Mycoplasma species, without dependence on tissue cultures free of Mycoplasma. (auth)
- Research Organization:
- Dept. of National Health and Welfare, Ottawa
- NSA Number:
- NSA-29-007930
- OSTI ID:
- 4368542
- Journal Information:
- Can. J. Microbiol., v. 19, no. 6, pp. 709-714, Other Information: Orig. Receipt Date: 30-JUN-74; Bib. Info. Source: CAN (Atomic Energy Commission Limited)
- Country of Publication:
- Canada
- Language:
- English
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