Host DNA synthesis-suppressing factor in culture fluid of tissue cultures infected with measles virus
Host DNA synthesis is suppressed by the culture fluid of cell cultures infected with measles virus. This activity in the culture fluid is initiated somewhat later than the growth of infectious virus. Ninety percent of host DNA synthesis in HeLa cells is inhibited by culture fluid of 3-day-old cell cultures of Vero or HeLa cells infected with measles virus. This suppressing activity is not a property of the virion, but is due to nonvirion-associated componentnent which shows none of the activities of measles virus such as hemagglutination, hemolysis, or cell fusion nor does it have the antigenicity of measles virus as tested by complement-fixation or hemagglutination-inhibiting antibody blocking tests. Neutralization of the activity of this component is not attained with the pooled sera of convalescent measles patients. This component has molecular weights of about 45,000, 20,000, and 3,000 and appears to be a heat-stable protein. The production of host DNA suppressing factor (DSF) is blocked by cycloheximide. Neither uv-inactivated nor antiserum-neutralized measles virus produce DSF. Furthermore, such activity of nonvirion-associated component is not detected in the culture fluid of cultures infected with other RNA viruses such as poliovirus, vesicular stomatitis virus, or Sindbis virus. (auth)
- Research Organization:
- Hokkaido Univ., Sapporo
- NSA Number:
- NSA-33-000883
- OSTI ID:
- 4173727
- Journal Information:
- J. Virol., v. 13, no. 5, pp. 118-125, Other Information: Orig. Receipt Date: 30-JUN-76
- Country of Publication:
- United States
- Language:
- English
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560131* -Biomedical Sciences
Applied Studies-Radiation Effects-Radiation Effects on Microorganisms-Basic Studies
*DNA- BIOSYNTHESIS
*HELA CELLS
*VIRUSES- BIOLOGICAL RADIATION EFFECTS
CYCLOHEXIMIDE
INHIBITION
MOLECULAR WEIGHT
TISSUE CULTURES
ULTRAVIOLET RADIATION