Inhibition of host cell RNA polymerase III-mediated transcription by poliovirus: Inactivation of specific transcription factors
The inhibition of transcription by RNA polymerase III in poliovirus-infected cells was studied. Experiments utilizing two different cell lines showed that the initiation step of transcription by RNA polymerase III was impaired by infection of these cells with the virus. The observed inhibition of transcription was not due to shut-off of host cell protein synthesis by poliovirus. Among four distinct components required for accurate transcription in vitro from cloned DNA templates, activities of RNA polymerase III and transcription factor TFIIIA were not significantly affected by virus infection. The activity of transcription factor TFIIIC, the limiting component required for transcription of RNA polymerase III genes, was severely inhibited in infected cells, whereas that of transcription factor TFIIIB was inhibited to a lesser extent. The sequence-specific DNA-binding of TFIIIC to the adenovirus VA1 gene internal promoted, however, was not altered by infection of cells with the virus. The authors conclude that (i) at least two transcription factors, TFIIIB and TFIIIC, are inhibited by infection of cells with poliovirtus, (ii) inactivation of TFIIIC does not involve destruction of its DNA-binding domain, and (iii) sequence-specific DNA binding by TFIIIC may be necessary but is not sufficient for the formation of productive transcription complexes.
- Research Organization:
- Dept. of Microbiology and Immunology and Jonsson Comprehensive Cancer Center, Univ. of California, Los Angeles, School of Medicine, and the Dept. of Microbiology and Molecular Biology Institute, Univ. of California, Los Angeles, CA 90024 (USA)
- OSTI ID:
- 7196494
- Journal Information:
- Mol. Cell. Biol.; (United States), Journal Name: Mol. Cell. Biol.; (United States) Vol. 7:11; ISSN MCEBD
- Country of Publication:
- United States
- Language:
- English
Similar Records
Resolution of human transcription factor TFIIIC into two functional components
Transcription factor IIIB generates extended DNA interactions in RNA polymerase III transcription complexes on tRNA genes
Related Subjects
550700 -- Microbiology
550900 -- Pathology
59 BASIC BIOLOGICAL SCIENCES
ADENOVIRUS
BIOLOGICAL PATHWAYS
CELL CULTURES
CELL TRANSFORMATIONS
DATA
DNA SEQUENCING
ENZYME INHIBITORS
ENZYMES
EXPERIMENTAL DATA
IN VITRO
INFORMATION
MICROORGANISMS
MOLECULAR BIOLOGY
NUCLEOPROTEINS
NUCLEOTIDYLTRANSFERASES
NUMERICAL DATA
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS-GROUP TRANSFERASES
POLIO VIRUS
POLYMERASES
PROTEINS
RNA POLYMERASES
SPECIFICITY
STRUCTURAL CHEMICAL ANALYSIS
TRANSCRIPTION
TRANSFERASES
VIRUSES