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Resolution of human transcription factor TFIIIC into two functional components

Journal Article · · Proc. Natl. Acad. Sci. U.S.A.; (United States)
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tRNA genes and adenovirus viral-associated (VA) genes are transcribed by RNA polymerase III. Transcription of these genes in vitro requires two protein fractions containing transcription factors designated TFIIIB and TFIIIC, in addition to RNA polymerase III. The authors report that the TFIIIC fraction derived from human cells in culture can be separated into two functional components, which they call TFIIIC1 and TFIIIC2. Both TFIIIC1 and TFIIIC2 fractions are required for in vitro transcription of the VA1 gene. In DNase I footprinting experiments, the TFIIIC2 fraction protects the internal control region termed the B block. Addition of the TFIIIC1 fractions extends the footprint over the internal control region called the A block. TFIIIC1 activity is the limiting transcription factor required for VA1 transcription in the crude extract. TFIIIC2 activity sediments as a large component of approx. = 18 S, while TFIIIC1 activity sediments at approx. = 9 S. These data indicate that the two activities are unique components and when added together reconstitute TFIIIC activity.

Research Organization:
Univ. of California, Los Angeles
OSTI ID:
5353940
Journal Information:
Proc. Natl. Acad. Sci. U.S.A.; (United States), Journal Name: Proc. Natl. Acad. Sci. U.S.A.; (United States) Vol. 84:11; ISSN PNASA
Country of Publication:
United States
Language:
English

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Related Subjects

550201 -- Biochemistry-- Tracer Techniques
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59 BASIC BIOLOGICAL SCIENCES
62 RADIOLOGY AND NUCLEAR MEDICINE
ADENOVIRUS
ANIMAL CELLS
ANIMALS
AUTORADIOGRAPHY
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOCHEMISTRY
CHEMISTRY
DAYS LIVING RADIOISOTOPES
ENZYMES
GENES
IN VITRO
ISOTOPES
LIGHT NUCLEI
MAMMALS
MAN
MICROORGANISMS
NUCLEI
NUCLEIC ACIDS
NUCLEOTIDYLTRANSFERASES
ODD-ODD NUCLEI
ONCOGENIC VIRUSES
ORGANIC COMPOUNDS
PARASITES
PHOSPHORUS 32
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
PRIMATES
PROTEINS
RADIOISOTOPES
RNA
RNA POLYMERASES
TRANSCRIPTION
TRANSFER RNA
TRANSFERASES
VERTEBRATES
VIRUSES