Radioimmunoassay for pituitary hormones

Journal Article · Sun Apr 01 04:00:00 EST 1973 · Rinsho Byori, v. 21, no. 4, pp. 293-297

OSTI ID:4365527

Miyai, K; Hashimoto, T; Azukizawa, M; Kumahara, Y

nese). Radioimmunoassays for thyroid-stimulating hormone (TSH), luteinizing hormone (LH), follicle-stimulating hormone (FSH) and adrenocorticotrophic hormone (ACTH) of anterior pituitary hormones were examined. TSH, FSH and LH were labeled with /sup 131 I/ by the Hunter and Greenwood method, and the resulting radioactivities were measured after separating bound and free hormones by the double antibody technique. As for the reproducibility of this method, the coefficient of variation was 18.3 to 23.3% for different assays for TSH and 6.5 to 9.0% for the same assay. The coefficient of variation in different assays for LH was 15% and for FSH was 17% and that in the same assay for both was 5%. The characteristics of this method were that the effects of other pituitary hormones on the measured hormone were few and the dilution curve of the serum was parallel to or coincided with the standard curve in the measurement of the serum samples. The recovery rate of /sup 131/I was 75 to 110%, when standard TSH was added to the serum of patients deficient in TSH. The levels of TSH, LH and FSH in the blood of patients with panhypopituitarism were less than the sensitivity of the measurement by this method. In primary hypothyroidism TSH level was high, and in primary hypogenitalism LH and FSH levels also were high. TSH in the blood increased after the administration of antithyroid agents and decreased after the administration of thyroid hormones. LH and FSH in the blood increased after the administration of gonadotropin. TSH in the blood increased markedly by the intravenous injection of 50 to 80 mu g TRH, which specifically secreted TSH, and LH and FSH in the blood increased by 100 mu g of LH releasing hormone. Characteristic variations of LH and FSH were concomitant with menstrual cycle variations. The measurement values by this method were well correlated with the values by bioassay, but the absolute values were higher for bioassay values, although the reason was unknown. (Japan)

• Research Organization: Osaka Univ.
• NSA Number: NSA-29-013061
• OSTI ID: 4365527
• Journal Information: Rinsho Byori, v. 21, no. 4, pp. 293-297, Journal Name: Rinsho Byori, v. 21, no. 4, pp. 293-297; ISSN RBYOA
• Country of Publication: Japan
• Language: Japanese

Related Subjects

*ACTH-- RADIOIMMUNOASSAY
*FSH-- RADIOIMMUNOASSAY
*LH-- RADIOIMMUNOASSAY
*TSH-- RADIOIMMUNOASSAY
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