In vitro and in vivo labeling of rabbit blood leukocytes with $sup 67$Ga- citrate

Journal Article · Fri Feb 01 04:00:00 EDT 1974 · J. Nucl. Med., v. 15, no. 2, pp. 98-101

OSTI ID:4351074

Burleson, R L; Johnson, M C; Head, H

The labeling characteristics of rabbit blood leukocytes with /sup 67/ Gacitrate, both in vitro and in vivo, have been defined. Both granulocytes and lymphocytes were consistently labeled by the in vitro incubation of anticoagulated whole blood with /sup 67/Ga-citrate, Minimal uptake by red blood cells occurred. Both the absolute and relative uptake of the radionuclide by granulocytes as compared with lymphocytes was greater in media-1640 than in anticoagulated whole blood. Maximal uptake of label by leukocytes occurred within 1 hr, was independent of temperature of incubation and type of anticoagulant, and occurred at a concentration of approximately 200 mu Ci /dup 67/Ga/10 ml of heparinized blood. Comparative studies with /sup 51/Cr demonstrated that /sup 67/Ga produced a label on both granulocytes and lymphocytes equal to or greater than the label obtained with /sup 67/Cr and that there was minimal loss of label by /sup 67/Ga-labeled cells with in vitro incubation. Blood leukocytes labeled in vitro with /sup 67/Ga offer an excellent gamma-emitting radioactive tracer that can be used to develop a technique for the scintigraphic detection and localization of abscesses. (auth)

• Research Organization: Walter Reed General Hospital, Washington, DC
• NSA Number: NSA-29-021294
• OSTI ID: 4351074
• Journal Information: J. Nucl. Med., v. 15, no. 2, pp. 98-101, Journal Name: J. Nucl. Med., v. 15, no. 2, pp. 98-101; ISSN JNMEA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

*GALLIUM 67-- UPTAKE
*LEUKOCYTES-- LABELLING
CITRATES
IN VITRO
IN VIVO
INCUBATION
N48830* --Life Sciences--New Tracer Techniques--Other
NUCLEAR MEDICINE
RABBITS
RADIOACTIVITY
TRACER TECHNIQUES