Mitotic consequences of radioinduced chromosome breaks in Lathyrus sativus and Vicia ervilia

Journal Article · Fri Sep 01 04:00:00 EDT 1972 · Cytologia (Tokyo), v. 37, no. 3, pp. 489-495

OSTI ID:4347325

Shaikh, M A.Q.; Godward, M B.E.

The mitotic consequences of gamma-induced chromosome breoks in L. sativus and V. ervilia following seed irradiation were studied. Various types of mitotic anomalies were encountered. These included single, double, triple, multiple, X-shaped, and interlocked bridges. The double bridges lasted very long persisting up to the late telophase and often left pear-shaped projections in the daughter nuclei marking their positions. Centric and acentric fragnnents in the metaphase and laggards in the anaphase were present. The fragnnents were of different lengths and the majority of them were in pairs. There were also some rings, minute deletions, dicentric chromosomes, and chromatids present. Unequal length of metaphase chromosomes showing much more variation in length than is observed in the normal karyotypes was observed. Condensed'' and noncondensed'' micronuclei in the interphase cells were observed and there was increasing evidence indicating that some of these micronuclei divide and go to the daughter nuclei. Degenerated cells having little chromatin material and showing less stainability were present. Giant cells often having about double the size of the ordinary root meristematic cells were present only in the species V. ervilia. Probable causes of the production of mitotic abnormalities following seed irradiation treatment are discussed in the light of the findings of other workers. (auth)

• Research Organization: Queen Mary Coll., London
• NSA Number: NSA-29-021138
• OSTI ID: 4347325
• Journal Information: Cytologia (Tokyo), v. 37, no. 3, pp. 489-495, Journal Name: Cytologia (Tokyo), v. 37, no. 3, pp. 489-495; ISSN CYTOA
• Country of Publication: Japan
• Language: English

Related Subjects

*CHROMOSOME BREAKAGE-- RADIOINDUCTION
*PEAS-- BIOLOGICAL RADIATION EFFECTS
*PLANT CELLS-- MITOSIS
*SEEDS-- BIOLOGICAL RADIATION EFFECTS
*VICIA-- BIOLOGICAL RADIATION EFFECTS
CHROMATIN
GAMMA RADIATION
GERMINATION
IRRADIATION
N48410* --Life Sciences--Radiation Effects on Plants--Basic Studies
RADIATION DOSES
ROOTS