Meiotic consequences of radioinduced chromosome breaks in Lathyrus sativus and Vicia ervilia

Journal Article · Fri Sep 01 04:00:00 EDT 1972 · Cytologia (Tokyo), v. 37, no. 3, pp. 497-505

OSTI ID:4347370

Shaikh, M A.Q.; Godward, M B.E.

Mature and dry seeds of L. satiwus and V. ervilia were irradiated with 5, 10, 15, 20, 25, 30, and 50 Krad doses of gamma rays (/sup 60/Co) and M/sub 1/ and M/sub 2/ plants were grown along with the con trols. Meiosis of both the generations were studied to detect and explain the types of chromosomal abnormalities produced at different stages of mciosis in the M/sub 1/ generation and that were subsequently still present in the meiosis of the M/ generation. Melosis in the control flowers of both the species was normal. The various types of abnormalities observed in the different stages of the M/sub 1/ generation were as follows: Diakinesis: heavy fragmentation of the nucleolus; metaphase 1: univalents, tetravalents, multivalents, fragments, complex interchanges, rings, clumped and unusual configurations, and unorientated chromatin material forming a large, round, and darkly stained mass when a few of the bivalents aligned at the equatorial plate; anaphase I: abnormal separation of the homologues, single and double bridges with or without fragments, anaphase I with laggards only, unequal numbers of chromosomes going to the poles; anaphase II: single bridges with or without laggards, anaphase II with laggards only, unequal volumes of chromatin material in daughter nuclei, unequal pollen grains, fused or twin pollen grains, and very small pollen grains. In the M/sub 2/ generation, very few meiotic aberrations were observed in both the species. They were as follows in the different stages of meiosis; diakinesis: fragmentation of the nucleolus; metaphase I: complex interchanges and configurations, fragments, unorientated chromatin material; anaphase I: lagging chromosomes in anophase I; anaphase II: laggards in anaphase II, and anequal volume of nuclear material in the daughter nuclei. (auth)

• Research Organization: Queen Mary Coll., London
• NSA Number: NSA-29-021136
• OSTI ID: 4347370
• Journal Information: Cytologia (Tokyo), v. 37, no. 3, pp. 497-505, Journal Name: Cytologia (Tokyo), v. 37, no. 3, pp. 497-505; ISSN CYTOA
• Country of Publication: Japan
• Language: English

Related Subjects

*CHROMOSOMAL ABERRATIONS-- RADIOINDUCTION
*PEAS-- BIOLOGICAL RADIATION EFFECTS
*PLANT CELLS-- MEIOSIS
*SEEDS-- BIOLOGICAL RADIATION EFFECTS
*VICIA-- BIOLOGICAL RADIATION EFFECTS
CHROMATIN
CHROMOSOMES
GAMMA RADIATION
IRRADIATION
N48410* --Life Sciences--Radiation Effects on Plants--Basic Studies
PHOTON BEAMS
PLANT GROWTH
POLLEN
RADIATION DOSES