Sequential study of phosphatase activity in beta-irradiated guinea-pig skin and its correlation with the histological changes
Histochemical studies are reported that were undertaken to detect alterations in hydrolase activity in BETA -irradiated guineapig skin and to correlate the enzyme changes with the histological changes during early and late tissue injury, and repair. No clearcut increase or decrease in acid phosphatase or appearance of alkaline phosphatase in the epidermal cells were found during the early period. Increased acid phosphatase activity began 10 days post- irradiation when epidermal necrosis had already appeared. The increase in enzyme activity became more marked as cytonecrosis became more advanced. At the same time, an intense alkaline phosphatase reaction made its appearance in the necrotic epidermal layers. Degenerating hair follicles showed an increase in acid phosphatase, whereas sebaceous glands also degenerated, but did so without noticeable change in enzyme reactivity. Regenerating epidermis showed some increase in acid phosphatase especially in the granular layer, but alkaline phosphatase was not demonstrable here, nor in normal epidermis. The enzyme 5'- nucleotidase normally present in the epidermis was absent after regeneration, Dermal fibroblasts responded promptly to irradiation by a transitory appearance of alkaline phosphatase and some increase in acid phosphatase during 24 hr post- irradiation. Later (10 to 17 days) the fibroblasts in the inflammatory or ulcerating regions stained intensely for both acid and alkaline phosphatases. During repair (13 to 22 days), the fibroblasts adjacent to the regenerating epidermis again showed a positive reaction for alkaline phosphatase and a moderate rise in acid phosphatase. These changes are thought to mean that, as might have been predicted, fibroblasts have an important role in skin repair after exposure to BETA radiation. (UK)
- Research Organization:
- Albert Einstein Medical Center, Philadelphia
- NSA Number:
- NSA-29-018628
- OSTI ID:
- 4341702
- Journal Information:
- J. Pathol., v. 110, no. 3, pp. 251-258, Other Information: Orig. Receipt Date: 30-JUN-74; Bib. Info. Source: UK (United Kingdom (sent to DOE from))
- Country of Publication:
- United Kingdom
- Language:
- English
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Related Subjects
*ACID PHOSPHATASE- EARLY RADIATION EFFECTS
*ALKALINE PHOSPHATASE- EARLY RADIATION EFFECTS
*EPIDERMIS- BIOLOGICAL REPAIR
*SKIN- RADIATION INJURIES
BETA PARTICLES
BIOLOGICAL REGENERATION
CATALYSIS
FIBROBLASTS
GLANDS
GUINEA PIGS
HAIR
LOCAL RADIATION EFFECTS
METABOLISM
NECROSIS
NUCLEOTIDASES