Radioresistance of anoxic cells and the effects of PNAP on the radiation response of an in vitro tumor model

Journal Article · Sat Dec 01 04:00:00 EST 1973 · Radiat. Res., v. 56, no. 3, pp. 460-473

DOI:https://doi.org/10.2307/3573716· OSTI ID:4339431

Hetzel, F W; Kruuv, J; Frey, H E; Koch, C J

Para-nitroacetophenone (PNAP) selectively sensitizes hypoxic mammalian cells to ionizing radiation. The growth of Chinese hamster V79 cells as multicellular spheroids provides a converient tumor model to test this drug. When irradiated, cells from these spheroids exhibit a multicomponent survival curve. The resistant portion of the curve is primarily due to hypoxic, noncycling cells near the center of the spheroid. However, radiation survival data indicate that these cells are as resistant as the most resistant cycling cells when the latt er are made hypoxic. Addition of 500 mu M PNAP prior to and during irradiation under oxic conditions decreases the survival on the resistant portion of the spheroid survival curve although the dose-modification is not as large as in hypoxic, monolayer systems. The analog, m-nitroacetophenone, acts in a similar fashion in spheroids. (auth)

• Research Organization: Univ. of Waterloo, Ont.
• Sponsoring Organization: USDOE
• NSA Number: NSA-29-015844
• OSTI ID: 4339431
• Journal Information: Radiat. Res., v. 56, no. 3, pp. 460-473, Journal Name: Radiat. Res., v. 56, no. 3, pp. 460-473; ISSN RAREA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

*ACETOPHENONE-- RADIOSENSITIVITY EFFECTS
*ANIMAL CELLS-- RADIOSENSITIVITY
*NITRO COMPOUNDS-- RADIOSENSITIVITY EFFECTS
*OXYGEN-- RADIOSENSITIVITY EFFECTS
ANOXIA
BIOLOGICAL MODELS
BIOLOGICAL RADIATION EFFECTS
CELL CULTURES
GAMMA RADIATION
HAMSTERS
IN VITRO
N48200* --Life Sciences--Radiation Effects on Cells
PHOTON BEAMS
RESPONSE MODIFYING FACTORS
SURVIVAL CURVES RADIOSENSITIVITY EFFECTS