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Radiation-induced DNA damage in V79 spheroids and monolayers. [. gamma. rays; cultured Chinese hamster (V79) cells]

Journal Article · · Radiat. Res.; (United States)
DOI:https://doi.org/10.2307/3575006· OSTI ID:6138181
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Chinese hamster V79 cells can be grown as monolayers or in suspension as multicell spheroids. When irradiated, cells from spheroids can accumulate more sublethal damage than cells from monolayers as evidenced by a wider shoulder on the survival curve. We have attempted to determine whether this increased resistance of spheroids to damage by ionizing radiation is reflected by DNA damage. The production and repair of single-strand breaks was quantified using alkaline sucrose gradient sedimentation and hydroxylapatite chromatography. Both methods indicated that equal amounts of radiation damage per rad were produced in cells grown as single cells or as spheroids, and that postirradiation rejoining of DNA single-strand breaks also occurred with similar efficiency. Thus, these results suggest that the enhanced survival of cells irradiated as spheroids is dependent on processes other than (or in addition to) initial radiation damage to DNA.
Research Organization:
Johns Hopkins Oncology Center, Baltimore, MD
OSTI ID:
6138181
Journal Information:
Radiat. Res.; (United States), Journal Name: Radiat. Res.; (United States) Vol. 78:1; ISSN RAREA
Country of Publication:
United States
Language:
English

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Related Subjects

550301 -- Cytology-- Tracer Techniques
560121* -- Radiation Effects on Cells-- External Source-- (-1987)
59 BASIC BIOLOGICAL SCIENCES
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ALKALI METAL ISOTOPES
ANIMAL CELLS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
BIOLOGICAL RECOVERY
BIOLOGICAL REPAIR
CELL CULTURES
CESIUM 137
CESIUM ISOTOPES
CULTIVATION TECHNIQUES
DATA
DATA FORMS
DNA
DOSE-RESPONSE RELATIONSHIPS
ELECTROMAGNETIC RADIATION
EXPERIMENTAL DATA
GAMMA RADIATION
GRAPHS
GROWTH
IN VITRO
INFORMATION
IONIZING RADIATIONS
IRRADIATION
ISOTOPES
NUCLEI
NUCLEIC ACIDS
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RADIOSENSITIVITY
RADIOSENSITIVITY EFFECTS
RECOVERY
REPAIR
STRAND BREAKS
SURVIVAL CURVES
SURVIVAL TIME
YEARS LIVING RADIOISOTOPES