LOCALIZATION OF CERIUM-144 IN THE SKELETAL TISSUES OF FETAL RATS

Technical Report · Fri Oct 18 00:00:00 EDT 1957

DOI:https://doi.org/10.2172/4332381· OSTI ID:4332381

Asling, C. W.; Johnston, M. E.; Durbin, P. W.; Hamilton, J. G.

The developing fetal skeleton of the rat was used to study the microscopic localization of the rare earth Ce/sup 144/ in bone. Nineteen-day-old rat fetuses were injected with Ce/sup 144/ in isotonic sodium citrate via the umbilical vein. They were sacrificed one-haif hour after the injection by immersion in 80% alcohol fixative. The fetuses were embedded in paraffin and sectioned at 10 to 12 mu . Semiserial sections were treated with periodic acid- Schiff stain for mucopolysaccharides, and by the von Kossa technique for calcium. Contract x-ray-film autoradiographs and NTB stripping-film autoradiographs were prepared. Photographs of representative sections and their respective autoradiographs are presented for various bones. This material strongly suggests that the initial binding site of Ce/sup 44/ in the skeleton is in the organic matrix of cartilage and bone, especially where this matrix is just entering the calcifiable state. This conclusion is highly dependent upon the specificity of the von Kossa stain for calcium. (auth)

• Research Organization: California Univ., Berkeley, CA (US). Radiation Lab.
• DOE Contract Number: W-7405-ENG-48
• NSA Number: NSA-12-013685
• OSTI ID: 4332381
• Report Number(s): UCRL-8024
• Country of Publication: United States
• Language: English

Related Subjects

AGE-- AMMONIUM COMPOUNDS-- BLOOD VESSELS-- BONES-- CERIUM 144-- CESIUM 137-- CITRIC ACID-- DISTRIBUTION-- FETUSES-- ION EXCHANGE-- MICROSCOPY-- MOLYBDENUM PHOSPHATES-- QUANTITATIVE ANALYSIS-- RAIN-- RATS-- SODIUM COMPOUNDS-- SOLUTIONS-- WATER
AMMONIUM COMPOUNDS-- BONES-- CALCIUM-- CERIUM 144-- CESIUM 137-- DISTRIBUTION-- DYES-- FILMS-- ION EXCHANGE-- IONIZATION-- MOLYBDENUM PHOSPHATES-- PHOTOGRAPHY-- PHYSIOLOGY-- QUANTITATIVE ANALYSIS-- RADIOAUTOGRAPHY-- RAIN-- TISSUES-- WATER
BIOLOGY AND MEDICINE