USE OF SERUM FROM IRRADIATED RABBITS AND GUINEA PIGS AS COMPLEMENT IN BACTERICIDAL REACTIONS
Journal Article
·
· J. Bacteriol
OSTI ID:4332231
The preparation of serum for use in complement fixation studies is simplified by the in vivo radioinduced inactivation of normal antibodies in the blood of donor animals. Normal bactericidins for Bacillus subtiles disappeared from the sera of rabbits and guinea pigs following exposure to doses of x radiation ranging from 200 to 1000 r. The optimum time for bleeding seemed to depend on the size of the animal. Results are presented for bactericidal tests with a normal mouse serum pool in the presence of complements from several sources. A more sensitive reaction was obtained with serum from irradiated animals. (C.H.)
- Research Organization:
- Univ. of Chicago
- NSA Number:
- NSA-12-011242
- OSTI ID:
- 4332231
- Journal Information:
- J. Bacteriol, Journal Name: J. Bacteriol Vol. Vol: 74, No. 6
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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