Genetic duplications induced at very high frequency by ultraviolet irradiation in Escherichia coli

Journal Article · Mon Jan 01 04:00:00 EST 1973 · Mol. Gen. Genet., v. 127, no. 3, pp. 197-214

DOI:https://doi.org/10.1007/BF00333760· OSTI ID:4325377

Hill, C W; Combriato, G

It was found that mild uitraviolet irradiation of Escherichia coli leads to a duplication of the gly T pur D region of the chromosome to the extent that 3 to 5% of all surviving chromosomes carry a genetic duplication of this material. The duplications vary in size from less than one to more than five minutes of the chromosome. While the endpoints of the duplications vary, seven of the characterized have one end between the pur D and met A loci, and five of these seven have the other endpoint near the arg H locus. Consequently, the region between pur D and met A (only 0.1 minutes) seems to be particularly prone to participating in abnormal recombinational events. The uv-induced damage leading to the genetic duplications is subject to dark repair, suggesting the involvement of pyrimidine dimers. Other mutagens such as nitrous acid, ethyl methanesulfonate, and nitrosoguanidine are also effective and probably most of the duplications are tandem duplications. However, at least one example was found that is more readily explained by a translocation. (auth)

• Research Organization: Pennsylvania State Univ., Hershey
• NSA Number: NSA-29-029700
• OSTI ID: 4325377
• Journal Information: Mol. Gen. Genet., v. 127, no. 3, pp. 197-214, Journal Name: Mol. Gen. Genet., v. 127, no. 3, pp. 197-214; ISSN MGGEA
• Country of Publication: Germany
• Language: English

Related Subjects

*ESCHERICHIA COLI-- GENETIC RADIATION EFFECTS
*GENE MUTATIONS-- RADIOINDUCTION
CHROMOSOMES
EMS
GENETIC EFFECTS
IRRADIATION
MUTAGENESIS
MUTAGENS
N48310* --Life Sciences--Radiation Effects on Microorganisms- -Basic Studies
NITROSO COMPOUNDS
NITROUS ACID
PHOTON BEAMS
ULTRAVIOLET RADIATION