Chemical radiosensitization of anoxic mammalian cells: effect of cell concentration

Journal Article · Fri Feb 01 04:00:00 EDT 1974 · Radiat. Res., v. 57, no. 2, pp. 246-259

DOI:https://doi.org/10.2307/3573831· OSTI ID:4310423

Agnew, D A; Skarsgard, L D

The effect of several electron affinic compounds on the radiation response of Chinese hamster cells irradiated in both dilute cell suspension and centrifugation pellets was investigated. p-Nitroacetophenone (500 mu M), 175 mu M nitrofurazone, and 200 mu M dimethyl fumarate, when present during the irradiation of anoxic, dilute cell suspensions, produced dose-modifying factors of 1.5, 1.8, and 1.8, respectively. Anoxic cells irradiated at high cell concentrations and in the presence of a sensitizer experienced a two-stage loss of much of the sensitization normally seen in dilute cell suspension. A large component of sensitization was lost upon formation of the cell pellet, reducirg the dose modifying factors to values approximately 70% of those obtained in dilute cell suspensions. This was followed by a slow disappearance of the remaining component of sensitization. Some possible mechanisms for this cell concentration effect are discussed. It is suggested that the cell pellet experiment may prove to be a useful indicator of the effectiveness of radiosensitizers in vivo. (auth)

• Research Organization: Univ. of British Columbia, Vancouver
• Sponsoring Organization: USDOE
• NSA Number: NSA-29-024427
• OSTI ID: 4310423
• Journal Information: Radiat. Res., v. 57, no. 2, pp. 246-259, Journal Name: Radiat. Res., v. 57, no. 2, pp. 246-259; ISSN RAREA
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

*AMIDES-- RADIOSENSITIVITY EFFECTS
*ANIMAL CELLS-- BIOLOGICAL RADIATION EFFECTS
*CARBOXYLIC ACID ESTERS-- RADIOSENSITIVITY EFFECTS
*DIAZO COMPOUNDS-- RADIOSENSITIVITY EFFECTS
*FURANS-- RADIOSENSITIVITY EFFECTS
*KETONES-- RADIOSENSITIVITY EFFECTS
*NITRO COMPOUNDS-- RADIOSENSITIVITY EFFECTS
*OXYGEN-- RADIOSENSITIVITY EFFECTS
*RADIOSENSITIZERS
ANOXIA
CELL CULTURES
GAMMA RADIATION
HAMSTERS
N48210* --Life Sciences--Radiation Effects on Cells-- External Source
PHOTON BEAMS
RESPONSE MODIFYING FACTORS RADIOSENSITIVITY EFFECTS