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EXPERIMENTS ON TRITIUM-LABELED INSULIN

Technical Report ·
OSTI ID:4293356

It is possible to label the proteohormone insu1in in high specific activity by means of the Wilzbach procedure with tritium. With this procedure the biological activity is preserved. Physicochemical and radiochemical properties of tritium--insulin are described. Estimation of the specific activity of amino acids chromatographically separated on columns gives insight into the exchangeability of hydrogen and tritium of the different amino acids bound in the protein. Results from animal experiments to test the tritium - insulin as a biological tracer are compared with those using iodine-131-labeled insulin. (C.H.)

Research Organization:
Univ. of Hamburg
NSA Number:
NSA-13-006136
OSTI ID:
4293356
Report Number(s):
A/CONF.15/P/979
Country of Publication:
Country unknown/Code not available
Language:
English

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