Radiochemical Purity of Tritium Exchange Labeled Barbital
Barbital (diethylmalonylurea) was isotopically labeled with tritium by the Wilzbach gas exposure procedure. The specific activity of the tritiated barbital was 266 mC/g after removal of labile tritium. The following criteria of homogeneity were employed: recrystallization to constant specific activity using 2 solvent systems; column liquid-liquid partition and paper chromatography; and evidence for stability after injection in rabbits. It was concluded that a product with a high degree of radiochemical purity and stability suitable for pharmacologic studies was obtained. After removal of labile tritium by exchange with water, the barbital-t retained approximates 96% of the remaining isotope through recrystallization and chromatography. This is the highest yield yet reported for the Wilzbach exchangelabeling method. Its final specific activity was 253 mC/g. This represents the presence of approximates 1 tritium atom per 610 molecules of barbital. The results are unusual since there was little evidence for the presence in the labeled material initially of a high specific activity contaminant.
- Research Organization:
- American Cyanamid Co., Pearl River, N.Y.
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-17-018136
- OSTI ID:
- 4743503
- Journal Information:
- Journal of Pharmaceutical Sciences, Journal Name: Journal of Pharmaceutical Sciences Journal Issue: 4 Vol. 51; ISSN 0022-3549
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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