EFFECT OF X-RADIATION ON DNA METABOLISM IN VARIOUS TISSUES OF THE RAT. I. INCORPORATION OF C$sup 14$-THYMIDINE INTO DNA DURING THE FIRST 24 HOURS POSTIRRADIATION

Journal Article · Wed Apr 01 04:00:00 EST 1959 · Radiation Research

DOI:https://doi.org/10.2307/3570837· OSTI ID:4281708

Nygaard, O F; Potter, R L

The effect of total-body x irradiation on the DNA synthesis in thymus, spleen, and small intestine of the rat has been studied throughout the first 24 hours after exposure to doses ranging from 50 to 800 r. The amounts of radioactivity, from thymidine-2-C/sup 14/, incorporated into DNA over 1-hour periods were used as a relative measure of synthesis. A rapid and extensive decrease in the incorporation of radioactivity was observed in all three tissues after the highest doses, with a gradually decreasing effect with lowering of the dose. In thymus and spleen there was no indication of recovery within the 24- hour period, whereas in the intestine there was an increase in labeling after 8 hours at all dose levels. The data obtained for the small intestine are compatible with the assumption that the decreased incorporation is, in part, caused by a delayed onset of DNA synthesis in some of the cells. This delay, at lesst at the earliest times after irradiation, is not the result of inhibition of mitosis. (auth)

• Research Organization: Univ. of Michigan, Ann Arbor
• Sponsoring Organization: USDOE
• NSA Number: NSA-13-010817
• OSTI ID: 4281708
• Journal Information: Radiation Research, Journal Name: Radiation Research Vol. Vol: 10
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

AMINO ACIDS
BIOLOGY AND MEDICINE
BODY
CARBON 14
CYTOLOGY
DNA
INTESTINE
LABELLED COMPOUNDS
MEASURED VALUES
METABOLISM
MITOSIS
NUCLEIC ACIDS
RADIATION DOSES
RADIATION EFFECTS
RADIOACTIVITY
RATS
RECOVERY
SPLEEN
THYMIDINE
THYMUS
VARIATIONS
X RADIATION