EFFECT OF RADIATION ON DNA METABOLISM IN VARIOUS TISSUES OF THE RAT. IV. EARLY EFFECTS

Journal Article · Thu Mar 01 04:00:00 EST 1962 · Radiation Research

DOI:https://doi.org/10.2307/3571156· OSTI ID:4835573

Nygaard, O F; Potter, R L

Radiation effects on the incorporation of thymidine-2-C/sup 14/ into DNA by spleen and small intestine were studied in the rat during the first 2 1/2 hours postirradiation. Relatively short exposures (l minute) as well as incorporation periods (S minutes) were employed in order to detect rapid and transient changes in the rate of labeling of DNA. The doses of irradiation ranged from l25 rep to 4600 rep of Co/sup 60/ gamma radiation and were given to the whole body. In both organs the incorporation of C/sup 14/ into DNA was immediately depressed. At the lower dose level a temporary reversal of this effect was observed, followed by a progressive and sustained drop in the incorporation. The effect of irradi ation on the phosphorylation of thymidine to thymine nucleotides in the spleen was not of a sufficient magnitude to account for the decreased rate of incorporation of C/sup 14/ inte the DNA of that organ. The results obtained do not permit any conclusion as to whether the immediate effect observed represents a direct effect on the process of DNA synthesis. in the case of the later postirradiation periods, delayed onset of DNA synthesis and cell death may account for the observed effects. (auth)

• Research Organization: Univ. of Michigan, Ann Arbor
• Sponsoring Organization: USDOE
• NSA Number: NSA-16-017510
• OSTI ID: 4835573
• Journal Information: Radiation Research, Journal Name: Radiation Research Vol. Vol: 16
• Country of Publication: Country unknown/Code not available
• Language: English

Related Subjects

ANIMAL CELLS
BIOCHEMISTRY
BIOLOGY AND MEDICINE
CARBON 14
COBALT 60
GAMMA RADIATION
INTESTINE
METABOLISM
NUCLEIC ACIDS
RADIATION DOSES
RADIATION EFFECTS
RATS
SPLEEN
TISSUES
TRACER TECHNIQUES