Interaction between cadmium and zinc in the production and sulfation of glycosaminoglycans in cultured bovine vascular endothelial cells

Journal Article · Fri Feb 09 04:00:00 EST 1996 · Journal of Toxicology and Environmental Health

DOI:https://doi.org/10.1080/009841096161889· OSTI ID:426082

Ohkawara, Susumu; Kaji, Toshiyuki; Yamamoto, Chika ^[1]

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1. Hokuriku Univ., Kanazawa (Japan); and others

Previously, we showed that cadmium stimulates the production of glycosaminoglycans (GAGs) but inhibits their sulfation in cultured bovine aortic endothelial cells. The effect of zinc on such alterations of GAGs induced by cadmium was investigated in the present study. The incorporation of [{sup 3}H]glucosamine and [{sup 35}S]sulfate into GAGs was determined by the cetylpyridinium chloride precipitation method as a marker of GAG production and GAG sulfation, respectively. The incorporation of both [{sup 3}H]glucosamine and [{sup 35}S]sulfate was not changed in GAGs accumulated in the endothelial cell layer and the conditioned medium after exposure to zinc at 20 {mu}M or less alone. A simultaneous exposure of the endothelial cell layer to zinc at 20 {mu}M or less and cadmium at 2{mu}M resulted in prevention of the cadmium-induced decrease in [{sup 35}S]sulfate incorporation; however, the cadmium-induced increase in [{sup 3}H]glucosamine incorporation was not affected by zinc. Characterization of GAGs in the cell layer revealed that such an interaction between zinc and cadmium occurred in both heparan sulfate and the other GAGs. Zinc significantly prevented the inhibition of either [{sup 3}H]thymidine or [{sup 3}H]leucine incorporation caused by cadmium with cadmium and protected endothelial cells from cadmium-induced inhibition of DNA and protein synthesis. The present data showed that a simultaneous exposure to cadmium and zinc resulted in an increase in heparan sulfate without a reduction of sulfation in the endothelial cell layer. The alteration may potentiate the antithrombogenic property of vascular endothelium. 30 refs., 2 figs., 3 tabs.

• OSTI ID: 426082
• Journal Information: Journal of Toxicology and Environmental Health, Journal Name: Journal of Toxicology and Environmental Health Journal Issue: 2 Vol. 47; ISSN 0098-4108; ISSN JTEHD6
• Country of Publication: United States
• Language: English

Related Subjects

56 BIOLOGY AND MEDICINE
APPLIED STUDIES
BIOLOGICAL EFFECTS
BIOLOGICAL PATHWAYS
CADMIUM
ENDOTHELIUM
GLUCOSAMINE
RESPONSE MODIFYING FACTORS
SULFATION
ZINC