INEFFECTIVENESS OF 1000 ROENTGENS IN VITRO ON THE APPARENT VIABILITY OF LYMPHOCYTES IN FRESHLY DRAWN BLOOD

Technical Report · Fri Aug 14 04:00:00 EDT 1959

OSTI ID:4256108

Cassen, B; Gutfreund, W

A technique developed for separating lymphocytes from freshly drawn whole blood was found to work well on rabbit's blood obtained by cardiac puncture. The separated lymphocytes showed typical ameboid motions 8 hours later when observed under a phase microscope. Samples of blood irradiated in vitro and receiving a dose of 1000 roentgens, showed after 6 hours the same viability as the controls. In each sample 25 lymphocytes were observed, almost all showing ameboid motions. Cell counts showed that lymphocytes were not lost by rapid lysis or by being phagocytized during irradiation. These observations are offered against hypotheses which explain the rapid decrease of lymphocyte count after total -body radiation on the basis of a direct lethal effect of the radiation on mature lymphocytes. It is not ruled out that they may be sublethally affected. If the lymphceytes are long lived cells, as appears probable from the results of several investigators, it would appear that new hypotheses are required to explain their rapid disappearance from the circulation after total-body radiation. (auth)

• Research Organization: California. Univ., Los Angeles. School of Medicine
• NSA Number: NSA-13-018804
• OSTI ID: 4256108
• Report Number(s): UCLA-445
• Country of Publication: United States
• Language: English

Related Subjects

BIOLOGY AND MEDICINE
BLOOD
BODY
CONTROL
HEART
LETHAL DOSE
LYMPHOCYTES
MICROSCOPY
RABBITS
RADIATION DOSES
SAMPLING
SURVIVAL TIME
X RADIATION