IRRADIATION OF LYMPHOCYTES IN FRESHLY DRAWN BLOOD
A technique developed for separating lymphocytes from freshly drawn whole blood wns found to work well on rabbit's blood obtained by cardiac puncture. The separated lymphocytes showed typinal ameboid motions as much as 8 hours later when observed under a phase microscope. Samples of blood irradiated in vitro and receiving a dose of 1000 r showed after 6 hours the same viability as the controls. In each sample 25 lymphocytes were observed, almost all showing ameboid motions. Cell courts showed that lymphocytes were not lost by rapid lysis or by being phagooytized during irradiation. On the assumption of the validity of experiments partially simulating in vivo factors, these observations are offered against hypotheses that explain the rapid decrease of lymphocyte count after totalbody irradiation on the basis of a rapid lethal effect of the radiation on matare lymphocytes. It is not ruled out that they may be sublethally affected. If the lymphocytes arp long-lived cells, as appears probable from the results of several investigators, it would appear that new hypotheses are required to explain the mechanism of their rapid disappparance from the circulation after total-body irradiation. (auth)
- Research Organization:
- Univ. of California, Los Angeles
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-14-021340
- OSTI ID:
- 4179602
- Journal Information:
- Radiation Research, Journal Name: Radiation Research Vol. Vol: 13
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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