ALPHA IRRADIATION OF PARTS OF SINGLE CELLS IN TISSUE CULTURE. III. IRRADIATION OF CHICK FIBROBLASTS DURING METAPHASE AND ANAPHASE
In order to investigate the relative importance of damage to nuclear material and cytoplasm when cells are exposed to ionizing radiations, parts of dividing chick fibroblasts in tissue cultures have been irradiated with alpha particles. The particles came from polonium deposited on the tip of a micro- needle; they had a well defined range, and accordingly, if the micro-needle was suitably positioned, a selected part of a cell could be irradiated. If small doses were delivered to the chromosomes at metaphase, sticky bridges were seen at anaphase. Larger doses would, in addition, inhibit nuclear reconstruction at telophase. Irradiation of one group of chromosomes during early anaphase with similar doses inhibited nuclear reconstruction in the corresponding daughter cell, but not in the unirradiated one. Irradiation of part of the cytoplasm and spindle at metaphase did not produce sticky bridges at anaphase, inhibit telophase, or induce any visible abnormality. The normal process of nuclear reconstruction at telophase in these cells is described. The clumped lateanaphase chromosomes spread, and the nuclear membrane first becomes visible, at a regular 8 minutes after the beginning of anaphase. The sensitivity to inhibition of nuclear reconstruction decreased sharply 5 to 6 minutes after anaphase had begun (i.e., 2 to 3 minutes before the nucleus was re-formed). (auth)
- Research Organization:
- Christie Hospital, Manchester, Eng.; and Holt Radium Inst., Manchester, Eng.
- Sponsoring Organization:
- USDOE
- NSA Number:
- NSA-13-021865
- OSTI ID:
- 4234342
- Journal Information:
- Exptl. Cell Research, Journal Name: Exptl. Cell Research Vol. Vol: 18
- Country of Publication:
- Country unknown/Code not available
- Language:
- English
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