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DETERMINATION OF IRRADIATION PRODUCTS BY MEANS OF PEROXIDATIVELY ACTING ENZYMES (in German)

Journal Article · · Helvetica Chimica Acta (Switzerland)
For the detection of H/sub 2/O/sub 2/ formed by x rays in aqueous solution two enzyme systems have been used: a) livercatalase and C/sup 14/ labeled formate, C/sup 14/O/sup 2/ being the reaction product; b) horseradish- peroxidase and 0-dianisidine, the oxidation of which can be followed colorimetrically. Liver-catalase and horseradish-peroxidase are resistant enough to yield amounts of reaction products directly proportional to the x-ray dose up to 15,000 r. The influence of experimental conditions and of inhibitors on the enzymatic component in radiation induced formate oxidation has been investigated and is discussed in view of the biological role of catalase. (auth)
Research Organization:
Universitat, Bern
NSA Number:
NSA-14-005230
OSTI ID:
4211500
Journal Information:
Helvetica Chimica Acta (Switzerland), Journal Name: Helvetica Chimica Acta (Switzerland) Journal Issue: 7 Vol. Vol: 42; ISSN HCACA
Country of Publication:
Country unknown/Code not available
Language:
German